ID   BU533588   standard; RNA; EST; 839 BP.
XX
AC   BU533588;
XX
SV   BU533588.1
XX
DT   14-SEP-2002 (Rel. 73, Created)
DT   16-SEP-2002 (Rel. 73, Last updated, Version 2)
XX
DE   AGENCOURT_10245849 NIH_MGC_126 Homo sapiens cDNA clone IMAGE:6560033 5',
DE   mRNA sequence.
XX
KW   EST.
XX
OS   Homo sapiens (human)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Primates; Catarrhini; Hominidae; Homo/Pan/Gorilla group; Homo.
XX
RN   [1]
RP   1-839
RA   NCI-MGC;
RT   "National Institutes of Health, Mammalian Gene Collection (MGC)
RT   http://mgc.nci.nih.gov/";
RL   Unpublished.
XX
CC   Contact: Robert Strausberg, Ph.D.
CC   Email: cgapbs-r@mail.nih.gov
CC   Tissue Procurement: NCI
CC    cDNA Library Preparation: Michael Brownstein Laboratory
CC    cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
CC    DNA Sequencing by: Agencourt Bioscience Corporation
CC    Clone distribution: MGC clone distribution information can be
CC   found through the I.M.A.G.E. Consortium/LLNL at:
CC   http://image.llnl.gov
CC   Plate: LLCM2732  row: d  column: 17
CC   High quality sequence stop: 528.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..839
FT                   /db_xref="taxon:9606"
FT                   /note="Vector: pDNR-LIB; Site_1: SfiI (ggccattatggcc);
FT                   Site_2: SfiI (ggccgcctcggcc); Double-stranded cDNA was
FT                   prepared from a pool of 40 cell line polyA+ RNAs (bladder -
FT                   2%, blood - 33.4%, brain - 5.6%, breast - 12.5%, colon -
FT                   4%, connective tissue - 1.4%, eye - 1%, intestine - 2.6%,
FT                   kidnney - 2.2%, liver - 5.7%, lung - 10.8%, NK-cell - 5.2%
FT                   , ovary - 4%, pharynx - 2.5%, prostate - 4.3%, salivary
FT                   gland - 1.3%, and skin - 2.3%).  5' and 3' adaptors were
FT                   used in cloning as follows:
FT                   5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' and
FT                   5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)NN-3'.  Full-length
FT                   enriched library was constructed using the Clontech Creator
FT                   SMART kit and size-selected to contain the 0.5-1 kb size
FT                   fraction (other fractions present in NIH_MGC_127 and
FT                   NIH_MGC_128).  Library created in the laboratory of T.
FT                   Usdin, M.D., Ph.D. (NIMH, NIH). Note: this is a NIH_MGC
FT                   Library."
FT                   /organism="Homo sapiens"
FT                   /clone="IMAGE:6560033"
FT                   /clone_lib="NIH_MGC_126"
FT                   /tissue_type="mixed (pool of 40 RNAs)"
FT                   /lab_host="DH10B (T1-phage-resistant)"
XX
SQ   Sequence 839 BP; 155 A; 278 C; 206 G; 200 T; 0 other;
     gggagggaaa cctggggcgt ctcgcgcaaa cgtccataac tgaaagtagc taaggcaccc        60
     cagccggagg aagtgagctc tcctggggcg tggttgttcg tgatccttgc atctgttact       120
     tagggtcaag gcttgggtct tgccccgcag acccttggga cgacccggcc ccagcgcagc       180
     tatgaacctg gagcgagtgt ccaatgagga gaaattgaac ctgtgccgga agtactacct       240
     gggggggttt gctttcctgc cttttctctg gttggtcaac atcttctggt tcttccgaga       300
     ggccttcctt gtcccagcct acacagaaca gagccaaatc aaaggctatg tctggcgctc       360
     agctgtgggc ttcctcttct gggtgatagt gctcacctcc tggatcacca tcttccagat       420
     ctaccggccc cgctggggtg cccttgggga ctacctctcc ttcaccatac ccctgggcac       480
     cccctgacaa cttctgcaca tactggggcc ctgcttattc tcccaggaca ggctccctta       540
     aagagaggaa ccctgtcctg ggcagccccc tctaaaactc ctaaaacctt ggtttccatg       600
     acccccgatc ctctgatgga catcccccca acaagggacc cctcactttc ctaccccacc       660
     ccctatagtg cccgatctta cactctttgt tggtatcccc cccgagcccg tcagataagg       720
     gtttcgccga ccagctttag ttttagtgtc cgcgcccccc tgtgaccgtc tcttgggccc       780
     ccgtggtaac catcgcctta agtgttgatg cgccccagcc tcccgccgta gcgaatcat        839
//