ID   BU646634   standard; RNA; EST; 417 BP.
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AC   BU646634;
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SV   BU646634.1
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DT   02-OCT-2002 (Rel. 73, Created)
DT   03-OCT-2002 (Rel. 73, Last updated, Version 2)
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DE   1112052D12.x1 C. reinhardtii CC-1690 (mt+), CC-1691 (mt-), Gamete
DE   (normalized), Lambda Zap II Chlamydomonas reinhardtii cDNA, mRNA sequence.
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KW   EST.
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OS   Chlamydomonas reinhardtii
OC   Eukaryota; Viridiplantae; Chlorophyta; Chlorophyceae; Volvocales;
OC   Chlamydomonadaceae; Chlamydomonas.
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RN   [1]
RP   1-417
RA   Grossman A., Chang C.-W., Davies J., Harris E., Hauser C., Lefebvre  P.,
RA   McDermott J.P., Shrager J., Silflow C., Stern D.;
RT   "Analyses of the Chlamydomonas reinhardtii Genome: A Model, Unicellular
RT   System for Analyzing Gene Function and Regulation in Vascular Plants.
RT   Project: 1112";
RL   Unpublished.
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CC   Contact: Charles Hauser
CC   DCMB Box 91000
CC   Duke University
CC   Durham, NC 27708-1000
CC   Tel: 919 613 8159
CC   Fax: 919 613 8177
CC   Email: chauser@duke.edu.
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FH   Key             Location/Qualifiers
FH
FT   source          1..417
FT                   /db_xref="taxon:3055"
FT                   /note="Vector: pBluescript II SK-; Site_1: EcoRI; Site_2:
FT                   XhoI; Gamete library, constructed by John Davies and
FT                   Jeffrey McDermott, combines cDNAs from both mating types of
FT                   strain 21gr which had been transfered to nitrogen-deficient
FT                   medium and sampled during the period of gametogenesis.
FT                   PolyA mRNA was purified from each sample, pooled and cDNA
FT                   synthesized. The cDNA was directionally cloned into lambda
FT                   Zap II (Stratagene) in the EcoRI (5') and XhoRI (3') sites.
FT                   pBluescript II SK- plasmids were excised from the lambda
FT                   ZAP clones by superinfection with ExAssist (Stratagene)
FT                   phage. The library was normalized using method 4 described
FT                   in Bonaldo et al., (1996) Genome Research 6: 791-806."
FT                   /organism="Chlamydomonas reinhardtii"
FT                   /strain="CC-1690 wild type mt+ and CC-1691 wild type mt-,
FT                   21gr"
FT                   /clone_lib="C. reinhardtii CC-1690 (mt+), CC-1691 (mt-),
FT                   Gamete (normalized), Lambda Zap II"
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SQ   Sequence 417 BP; 97 A; 116 C; 100 G; 104 T; 0 other;
     ttttttctgg ccgtagtgtt tacatgtgat atactggccg aagaggcttg agtagctctc        60
     ggcctcacga tgcctagtcc ctcagcaagg gctccatgct tgcaagcatg ctgggacagc       120
     ctgaccacac agcatcaatg agtgtgcacg catcaacaat caacattact atgtccattt       180
     atgtgagagt ccttggcgcg ctatgaatgc gctggatgcc ttatgtcttc tgcgcaaagg       240
     gcccatccaa cgtatctata cattgggccc cagtagcgcg cacagaagtg tcaacggaac       300
     gcaactccgt gcgagtctta tgcggtagtc caagggcagc gctgtccgcg ctagatattc       360
     tgctatgtat gtaatctagc gcctttacta acggcgcctc cacacctaca aacccta          417
//