ID   BU650578   standard; RNA; EST; 308 BP.
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AC   BU650578;
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SV   BU650578.1
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DT   02-OCT-2002 (Rel. 73, Created)
DT   03-OCT-2002 (Rel. 73, Last updated, Version 2)
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DE   1112087H05.x1 C. reinhardtii CC-1690 (mt+), CC-1691 (mt-), Gamete
DE   (normalized), Lambda Zap II Chlamydomonas reinhardtii cDNA, mRNA sequence.
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KW   EST.
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OS   Chlamydomonas reinhardtii
OC   Eukaryota; Viridiplantae; Chlorophyta; Chlorophyceae; Volvocales;
OC   Chlamydomonadaceae; Chlamydomonas.
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RN   [1]
RP   1-308
RA   Grossman A., Chang C.-W., Davies J., Harris E., Hauser C., Lefebvre  P.,
RA   McDermott J.P., Shrager J., Silflow C., Stern D.;
RT   "Analyses of the Chlamydomonas reinhardtii Genome: A Model, Unicellular
RT   System for Analyzing Gene Function and Regulation in Vascular Plants.
RT   Project: 1112";
RL   Unpublished.
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CC   Contact: Charles Hauser
CC   DCMB Box 91000
CC   Duke University
CC   Durham, NC 27708-1000
CC   Tel: 919 613 8159
CC   Fax: 919 613 8177
CC   Email: chauser@duke.edu.
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FH   Key             Location/Qualifiers
FH
FT   source          1..308
FT                   /db_xref="taxon:3055"
FT                   /note="Vector: pBluescript II SK-; Site_1: EcoRI; Site_2:
FT                   XhoI; Gamete library, constructed by John Davies and
FT                   Jeffrey McDermott, combines cDNAs from both mating types of
FT                   strain 21gr which had been transfered to nitrogen-deficient
FT                   medium and sampled during the period of gametogenesis.
FT                   PolyA mRNA was purified from each sample, pooled and cDNA
FT                   synthesized. The cDNA was directionally cloned into lambda
FT                   Zap II (Stratagene) in the EcoRI (5') and XhoRI (3') sites.
FT                   pBluescript II SK- plasmids were excised from the lambda
FT                   ZAP clones by superinfection with ExAssist (Stratagene)
FT                   phage. The library was normalized using method 4 described
FT                   in Bonaldo et al., (1996) Genome Research 6: 791-806."
FT                   /organism="Chlamydomonas reinhardtii"
FT                   /strain="CC-1690 wild type mt+ and CC-1691 wild type mt-,
FT                   21gr"
FT                   /clone_lib="C. reinhardtii CC-1690 (mt+), CC-1691 (mt-),
FT                   Gamete (normalized), Lambda Zap II"
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SQ   Sequence 308 BP; 59 A; 117 C; 72 G; 60 T; 0 other;
     ttttttcctg tctttctgct tttacacacg cccgctgcct gctgctgctg ccgccacaca        60
     ctaggcggat cactcgatgc cgcacgtgac acgcctgtcc catgacacag cacacagcgc       120
     ctgtgcccct cagcagcgcc cccaccaggc gcacttggca caagccaaca gcagttgccc       180
     gggccctcta acataagcag caatttgatc tacacgtcat aaacttgtgc agtcctgtgg       240
     tcgtgcgtcc gggcactggg gacttgcctg gacgcacacc gacccgcacg ccgtggcccc       300
     cacacaac                                                                308
//