ID   BU096441   standard; RNA; EST; 309 BP.
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AC   BU096441;
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SV   BU096441.1
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DT   20-NOV-2002 (Rel. 73, Created)
DT   20-NOV-2002 (Rel. 73, Last updated, Version 1)
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DE   tca-926 tca Trypanosoma carassii cDNA clone 05f8 5', mRNA sequence.
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KW   EST.
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OS   Trypanosoma carassii
OC   Eukaryota; Euglenozoa; Kinetoplastida; Trypanosomatidae; Trypanosoma.
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RN   [1]
RP   1-309
RA   Aguero F., Campo V., Cremona L., Jager A., Di Noia J.M., Overath P. ,
RA   Sanchez D.O., Frasch A.C.;
RT   "Gene discovery in the freshwater fish parasite Trypanosoma carassii:
RT   identification of trans-sialidase-like and mucin-like genes";
RL   Infect. Immun. 70(12):7140-7144(2002).
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CC   Contact: Sanchez DO
CC   Genomics and Bioinformatics
CC   Instituto de Investigaciones Biotecnologicas
CC   Parque Tecnologico Miguelete, INTI, Edificio 24, 1650, San Martin,
CC   Buenos Aires, Argentina
CC   Tel: (54-11) 4580-7255
CC   Fax: (54-11) 4752-9639
CC   Email: dsanchez@iib.unsam.edu.ar
CC   Sequences were basecalled with phred and vector was masked with
CC   crossmatch (see http://www.phrap.org). Sequences were then trimmed
CC   from both ends to remove low quality bases and masked vector.
CC   Plate: 05  row: f  column: 8
CC   Seq primer: T7.
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FH   Key             Location/Qualifiers
FH
FT   source          1..309
FT                   /db_xref="taxon:38249"
FT                   /note="Vector: pSport1; Blood trypomastigotes were obtained
FT                   from goldfish and cultured as described (Overath et al.
FT                   Parasitol Res (1998) 84:343) before obtaining total RNA
FT                   using TRIzol. cDNA library construction was made from
FT                   polyA+ mRNA using a poly-dT oligonucleotide as primer. The
FT                   cDNAs were cloned in a oriented manner using a commercial
FT                   kit (SuperScript Plasmid System for cDNA Synthesis and
FT                   Plasmid Cloning, Life Technologies)."
FT                   /organism="Trypanosoma carassii"
FT                   /clone="05f8"
FT                   /clone_lib="tca"
FT                   /dev_stage="blood trypomastigote"
FT                   /lab_host="Goldfish (Carassius auratus)"
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SQ   Sequence 309 BP; 94 A; 46 C; 55 G; 114 T; 0 other;
     ccacgcgtcc ggggtaattt attgtcttga atcaaaaaaa ttatatatat atatatatat        60
     gtgtgtgtat atataatatt tttttttgac gatgagggca taccatattt attttagtat       120
     agtgattcgt tttttgttac gtgttgaaaa gtaacaaaaa gagggcctat atatatatat       180
     atatattgag agcttgcgac atgaatggta ttacctgact gtttcttgat ttaaactttt       240
     tcttattaag atataaaggg cgtctcgaga agtaagtttc tgaaaaaccc ggcctacccg       300
     ccccctccc                                                               309
//