ID   BU096576   standard; RNA; EST; 220 BP.
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AC   BU096576;
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SV   BU096576.1
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DT   20-NOV-2002 (Rel. 73, Created)
DT   20-NOV-2002 (Rel. 73, Last updated, Version 1)
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DE   tca-1061 tca Trypanosoma carassii cDNA clone 05o15 5', mRNA sequence.
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KW   EST.
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OS   Trypanosoma carassii
OC   Eukaryota; Euglenozoa; Kinetoplastida; Trypanosomatidae; Trypanosoma.
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RN   [1]
RP   1-220
RA   Aguero F., Campo V., Cremona L., Jager A., Di Noia J.M., Overath P. ,
RA   Sanchez D.O., Frasch A.C.;
RT   "Gene discovery in the freshwater fish parasite Trypanosoma carassii:
RT   identification of trans-sialidase-like and mucin-like genes";
RL   Infect. Immun. 70(12):7140-7144(2002).
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CC   Contact: Sanchez DO
CC   Genomics and Bioinformatics
CC   Instituto de Investigaciones Biotecnologicas
CC   Parque Tecnologico Miguelete, INTI, Edificio 24, 1650, San Martin,
CC   Buenos Aires, Argentina
CC   Tel: (54-11) 4580-7255
CC   Fax: (54-11) 4752-9639
CC   Email: dsanchez@iib.unsam.edu.ar
CC   Sequences were basecalled with phred and vector was masked with
CC   crossmatch (see http://www.phrap.org). Sequences were then trimmed
CC   from both ends to remove low quality bases and masked vector.
CC   Plate: 05  row: o  column: 15
CC   Seq primer: T7.
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FH   Key             Location/Qualifiers
FH
FT   source          1..220
FT                   /db_xref="taxon:38249"
FT                   /note="Vector: pSport1; Blood trypomastigotes were obtained
FT                   from goldfish and cultured as described (Overath et al.
FT                   Parasitol Res (1998) 84:343) before obtaining total RNA
FT                   using TRIzol. cDNA library construction was made from
FT                   polyA+ mRNA using a poly-dT oligonucleotide as primer. The
FT                   cDNAs were cloned in a oriented manner using a commercial
FT                   kit (SuperScript Plasmid System for cDNA Synthesis and
FT                   Plasmid Cloning, Life Technologies)."
FT                   /organism="Trypanosoma carassii"
FT                   /clone="05o15"
FT                   /clone_lib="tca"
FT                   /dev_stage="blood trypomastigote"
FT                   /lab_host="Goldfish (Carassius auratus)"
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SQ   Sequence 220 BP; 79 A; 25 C; 53 G; 63 T; 0 other;
     ccacgcgtcc gttttttgag ttttgggttt tactttgttt ggtgagaaag agcccacttg        60
     agcccagcag gaatcgacga ttttaagctt tctggatttt aaaaggaaaa cgggtggagg       120
     gaaacgaaaa gaatgggggg agaacccgtg atttatatat tgtgtatacg agatagttaa       180
     aattaaaata taaatgtttt taaaaaaaaa aaaaaaaaag                             220
//