ID   CA589893   standard; RNA; EST; 490 BP.
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AC   CA589893;
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SV   CA589893.1
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DT   20-NOV-2002 (Rel. 73, Created)
DT   20-NOV-2002 (Rel. 73, Last updated, Version 1)
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DE   hab39f05.y1 Fugu UT6 adult gut Takifugu rubripes cDNA clone IMAGE:6351104
DE   5' similar to SW:MPK4_MOUSE P47809 DUAL SPECIFICITY MITOGEN-ACTIVATED
DE   PROTEIN KINASE KINASE 4 ;, mRNA sequence.
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KW   EST.
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OS   Takifugu rubripes
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Euteleostei; Neoteleostei;
OC   Acanthomorpha; Acanthopterygii; Percomorpha; Tetraodontiformes;
OC   Tetraodontidae; Takifugu.
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RN   [1]
RP   1-490
RA   Clark M., Johnson S.L., Lehrach H., Lee R., Li F., Marra M., Eddy  S.,
RA   Hillier L., Kucaba T., Martin J., Beck C., Wylie T., Underwood  K.,
RA   Steptoe M., Theising B., Allen M., Bowers Y., Person B., Swaller T.,
RA   Gibbons M., Pape D., Harvey N., Schurk R., Ritter E., Kohn S., Shin T.,
RA   Jackson Y., Cardenas M., McCann R., Waterston R., Wilson R.;
RT   "WashU Zebrafish EST Project 1998";
RL   Unpublished.
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CC   Other_ESTs: hab39f05.x1
CC   Contact: Stephen L. Johnson
CC   Washington University School of Medicine
CC   4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
CC   Tel: 314 286 1800
CC   Fax: 314 286 1810
CC   Email: zbrafish@watson.wustl.edu
CC   Library materials provided by G. Elgar (UK MRC HGMP-RC) Library
CC   constructed by Drs. K. Kawakami, M. Sasaki, S. Sugano, K. Kikuchi
CC   and S. Watabe (University of Tokyo, Institute of Medical Science
CC   and Laboratory of Aquatic Molecular Biology and Biotechnology) DNA
CC   Sequencing by: Washington University Genome Sequencing Center Clone
CC   distribution: Fugu clone distribution information can be found
CC   through the I.M.A.G.E. Consortium/LLNL, send email to:
CC   info@image.llnl.gov
CC   Seq primer: T3 ET from Amersham.
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FH   Key             Location/Qualifiers
FH
FT   source          1..490
FT                   /db_xref="taxon:31033"
FT                   /note="Vector: pBluescript-FL; Site_1: PflM I (CCANNNNNTGG
FT                   ); Site_2: PflM I (CCANNNNNTGG); BamHI-SmaI sites were
FT                   converted to BamHI-PflMI-SfiI-PflMI sites (SmaI is
FT                   destroyed). Other part of the vector is untouched. The cDNA
FT                   is inserted between two PflMI sites in T3 (5') to T7 (3')
FT                   direction. Library materials provided by G. Elgar (UK MRC
FT                   HGMP-RC) and constructed and donated by Drs. K. Kawakami,
FT                   M. Sasaki, S. Sugano, K. Kikuchi and S. Watabe (University
FT                   of Tokyo, Institute of Medical Science and Laboratory of
FT                   Aquatic Molecular Biology and Biotechnology )."
FT                   /organism="Takifugu rubripes"
FT                   /clone="IMAGE:6351104"
FT                   /clone_lib="Fugu UT6 adult gut"
FT                   /tissue_type="gut"
FT                   /dev_stage="adult"
FT                   /lab_host="DH10B (T1 phage-resistant)"
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SQ   Sequence 490 BP; 118 A; 160 C; 131 G; 81 T; 0 other;
     ttagccagcg tcgttagctt agctgcatgt ttgtggtcgt cagcgttcgc agaggaagct        60
     tccgaaaggt cgacgcatct cgctggtatc gaaccagaac ctccccgcta agcggctgtg       120
     ttgcggcccg aagctgccct cgctgaggca ggagcgacgg tgggaaggtg aacttgatct       180
     ccgcgtatct gcattgcagc ccggcgctgc tgatggaatt caagcacgca acaatggcga       240
     gttccaacaa ctcttcatcc cccaacggcc acaacagcag cggcagcggg ccggcggcga       300
     accaccagca ccagtcccag cacatcacca ccatgagcag cccccaggac tcgaacacct       360
     gctggagatg tcagagcgaa acaggtaaac gtaaagccct gaagctgaac tttgccaacc       420
     ctccgatcaa acccatgacc aggttcacgc tgaacacggc gggcccgccc ttccagaacc       480
     cgcacataga                                                              490
//