ID   BU095524   standard; RNA; EST; 337 BP.
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AC   BU095524;
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SV   BU095524.1
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DT   20-NOV-2002 (Rel. 73, Created)
DT   20-NOV-2002 (Rel. 73, Last updated, Version 1)
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DE   tca-9 tca Trypanosoma carassii cDNA clone 01a3 5', mRNA sequence.
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KW   EST.
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OS   Trypanosoma carassii
OC   Eukaryota; Euglenozoa; Kinetoplastida; Trypanosomatidae; Trypanosoma.
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RN   [1]
RP   1-337
RA   Aguero F., Campo V., Cremona L., Jager A., Di Noia J.M., Overath P. ,
RA   Sanchez D.O., Frasch A.C.;
RT   "Gene discovery in the freshwater fish parasite Trypanosoma carassii:
RT   identification of trans-sialidase-like and mucin-like genes";
RL   Infect. Immun. 70(12):7140-7144(2002).
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CC   Contact: Sanchez DO
CC   Genomics and Bioinformatics
CC   Instituto de Investigaciones Biotecnologicas
CC   Parque Tecnologico Miguelete, INTI, Edificio 24, 1650, San Martin,
CC   Buenos Aires, Argentina
CC   Tel: (54-11) 4580-7255
CC   Fax: (54-11) 4752-9639
CC   Email: dsanchez@iib.unsam.edu.ar
CC   Sequences were basecalled with phred and vector was masked with
CC   crossmatch (see http://www.phrap.org). Sequences were then trimmed
CC   from both ends to remove low quality bases and masked vector.
CC   Plate: 01  row: a  column: 3
CC   Seq primer: T7.
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FH   Key             Location/Qualifiers
FH
FT   source          1..337
FT                   /db_xref="taxon:38249"
FT                   /note="Vector: pSport1; Blood trypomastigotes were obtained
FT                   from goldfish and cultured as described (Overath et al.
FT                   Parasitol Res (1998) 84:343) before obtaining total RNA
FT                   using TRIzol. cDNA library construction was made from
FT                   polyA+ mRNA using a poly-dT oligonucleotide as primer. The
FT                   cDNAs were cloned in a oriented manner using a commercial
FT                   kit (SuperScript Plasmid System for cDNA Synthesis and
FT                   Plasmid Cloning, Life Technologies)."
FT                   /organism="Trypanosoma carassii"
FT                   /clone="01a3"
FT                   /clone_lib="tca"
FT                   /dev_stage="blood trypomastigote"
FT                   /lab_host="Goldfish (Carassius auratus)"
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SQ   Sequence 337 BP; 109 A; 54 C; 63 G; 111 T; 0 other;
     ccacgcgtcc ggcaaaagtt gcaacgcggt ttgagccttc agggatgatg tttcgcaaag        60
     gatgtctgga aaagtggtat aaaaaatcaa gacaacacaa tggcttattt tgacatttgt       120
     ttgtcaagca gcttttttaa aaaatatctt gcaagaatta tcaagtcatc tctttttttt       180
     ttcataactt tgcaacaagt gtgaagcaaa atttctgtgc ctttgagggg catagcatct       240
     tttgaatata aacactttga aagtatgttt aaataataaa gcaaaaggag gacacacaat       300
     tttttaattt gtgcgccttg gtcttctctt taaaaaa                                337
//