ID   BH917308   standard; DNA; GSS; 222 BP.
XX
AC   BH917308;
XX
SV   BH917308.1
XX
DT   14-SEP-2002 (Rel. 73, Created)
DT   15-SEP-2002 (Rel. 73, Last updated, Version 2)
XX
DE   3526_1_55_1_E12.1EL_y_1 3526 - RescueMu Grid K Zea mays genomic, DNA
DE   sequence.
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KW   GSS.
XX
OS   Zea mays
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; PACC clade;
OC   Panicoideae; Andropogoneae; Zea.
XX
RN   [1]
RP   1-222
RA   Walbot V.;
RT   "Maize genomic sequences found using engineered RescueMu transposon";
RL   Unpublished.
XX
CC   Contact: Walbot V
CC   Department of Biological Sciences
CC   Stanford University
CC   855 California Ave, Palo Alto, CA 94304, USA
CC   Tel: 650 723 2227
CC   Fax: 650 725 8221
CC   Email: walbot@stanford.edu
CC   Very probable ligation site of ends cut by single endonuclease.
CC   Reverse complemented post-ligation sequence from source sequence.
CC   Plate: 3526_1_55_1  row: 19
CC   Class: transposon-tagged.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..222
FT                   /db_xref="taxon:4577"
FT                   /note="Organ: leaf; Vector: RescueMu (engineered from
FT                   pBlueScript backbone); Site_1: BamHI; Site_2: BglII;
FT                   RescueMu is a 4.9 kb, modified maize Mu transposon designed
FT                   to allow plasmid rescue from total genomic DNA. Mu elements
FT                   insert preferentially into transcription units. For more
FT                   information on RescueMu, go to the web site
FT                   'www.zmdb.iastate.edu' and follow the links for 'RescueMu.'
FT                   Grid K was grown at Molokai, Hawaii in Winter 2000-2001.
FT                   DNA was extracted from leaf punches, double digested using
FT                   BamHI and BglII, and ligated to form circular plasmids.
FT                   DH10B cells were transformed and then screened on LB plates
FT                   with ampicillin."
FT                   /organism="Zea mays"
FT                   /cultivar="mixed background W23/A188/B73"
FT                   /clone_lib="3526 - RescueMu Grid K"
FT                   /tissue_type="leaf"
FT                   /dev_stage="adult"
FT                   /lab_host="DH10B"
XX
SQ   Sequence 222 BP; 52 A; 59 C; 63 G; 48 T; 0 other;
     tgtcaccagt cccattcact gcctcaagca aggctgaggc gacatgccgc gaacaaagag        60
     tcctggccag cgcgagcgcg acggccggaa cgtacgtata gcgaaccatg gattcagacc       120
     ctggagtgga gaaccaggta ctgcagcagg ttcgttgcat tcgattcagg cttggacatg       180
     tacatacatg gttttggttc aggctgactc ctattctgga tc                          222
//