ID   BU501321   standard; RNA; EST; 993 BP.
XX
AC   BU501321;
XX
SV   BU501321.1
XX
DT   14-SEP-2002 (Rel. 73, Created)
DT   16-SEP-2002 (Rel. 73, Last updated, Version 2)
XX
DE   AGENCOURT_8877539 NIH_MGC_43 Homo sapiens cDNA clone IMAGE:6338027 5', mRNA
DE   sequence.
XX
KW   EST.
XX
OS   Homo sapiens (human)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Primates; Catarrhini; Hominidae; Homo/Pan/Gorilla group; Homo.
XX
RN   [1]
RP   1-993
RA   NCI-MGC;
RT   "National Institutes of Health, Mammalian Gene Collection (MGC)
RT   http://mgc.nci.nih.gov/";
RL   Unpublished.
XX
CC   Contact: Robert Strausberg, Ph.D.
CC   Email: cgapbs-r@mail.nih.gov
CC   Tissue Procurement: ATCC
CC    cDNA Library Preparation: Rubin Laboratory
CC    cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
CC    DNA Sequencing by: Agencourt Bioscience Corporation
CC    Clone distribution: MGC clone distribution information can be
CC   found through the I.M.A.G.E. Consortium/LLNL at:
CC   http://image.llnl.gov
CC   Plate: LLCM2530  row: j  column: 12
CC   High quality sequence stop: 522.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..993
FT                   /db_xref="taxon:9606"
FT                   /note="Organ: eye; Vector: pOTB7; Site_1: XhoI; Site_2:
FT                   EcoRI; cDNA made by oligo-dT priming.  Directionally cloned
FT                   into EcoRI/XhoI sites using the following 5' adaptor:
FT                   GGCACGAG(G).  Library constructed  by Ling Hong in the
FT                   laboratory of Gerald M. Rubin (University of California,
FT                   Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and 
FT                   Superscript II RT (Life Technologies). Note: this is a
FT                   NIH_MGC Library. |"
FT                   /organism="Homo sapiens"
FT                   /clone="IMAGE:6338027"
FT                   /clone_lib="NIH_MGC_43"
FT                   /tissue_type="normal pigmented retinal epithelium"
FT                   /lab_host="DH10B (phage-resistant)"
XX
SQ   Sequence 993 BP; 161 A; 341 C; 298 G; 193 T; 0 other;
     cgacgcaaac atggcagcgc agaaggacca gcagaaagat gccgaggcgg aagggctgag        60
     cggcacgacc ctgctgccga agctgattcc ctccggtgca ggccgggagt ggctggagcg       120
     gcgccgcgcg accatccggc cctggagcac cttcgtggac cagcagcgct tctcacggcc       180
     ccgcaacctg ggagagctgt gccagcgcct cgtacgcaac gtggagtact accagagcaa       240
     ctatgtgttc gtgttcctgg gcctcatcct gtactgtgtg gtgacgtccc ctatgttgct       300
     ggtggctctg gctgtctttt tcggcgcctg ttacattctc tatctgcgca ccttggagtc       360
     caagcttgtg ctctttggcc gagaggtgag cccagcgcat cagtatgctc tggctggagg       420
     catctccttc cccttcttct ggctggctgg tgcgggctcg gccgtcttct gggtgctggg       480
     agccaccctg gtggtcatcg gctcccacgc ctgccttcca ccagattgag gctgtggacg       540
     gggaggagct gcatatggaa cccgtgtgag gtgtcttctg ggacctgccg gcctcccggg       600
     gcagctgacc cacccctgcc catgcctgtc ctgcacggcc ttctgctgct cgggccccca       660
     cagcggccgt cccatcaaca agccccgggg ggagggaatc ccgcccctct gaaaaacaaa       720
     cgctggttat tggggccggg cctcccccgg caaatccggc ggagccagaa tgcgcaacac       780
     cctccgacgg cacttccagg tttcggcgcg gccgggacaa aaaaggggca atggccacac       840
     ggggcccact ggggtcccca taactctggc tttccccgga cccacaccct ttttccccgt       900
     ggtcacccaa gacggggggc gagaacttcc gagccggggg ttctcccctc ctaaatatcc       960
     ctgacccccc ccctcacttc ctaaatctct ccg                                    993
//