ID   BU506699   standard; RNA; EST; 867 BP.
XX
AC   BU506699;
XX
SV   BU506699.1
XX
DT   14-SEP-2002 (Rel. 73, Created)
DT   16-SEP-2002 (Rel. 73, Last updated, Version 2)
XX
DE   AGENCOURT_10036486 NIH_MGC_142 Homo sapiens cDNA clone IMAGE:6498699 5',
DE   mRNA sequence.
XX
KW   EST.
XX
OS   Homo sapiens (human)
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Primates; Catarrhini; Hominidae; Homo/Pan/Gorilla group; Homo.
XX
RN   [1]
RP   1-867
RA   NCI-MGC;
RT   "National Institutes of Health, Mammalian Gene Collection (MGC)
RT   http://mgc.nci.nih.gov/";
RL   Unpublished.
XX
CC   Contact: Robert Strausberg, Ph.D.
CC   Email: cgapbs-r@mail.nih.gov
CC   Tissue Procurement: NCI
CC    cDNA Library Preparation: Michael Brownstein Laboratory
CC    cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
CC    DNA Sequencing by: Agencourt Bioscience Corporation
CC    Clone distribution: MGC clone distribution information can be
CC   found through the I.M.A.G.E. Consortium/LLNL at:
CC   http://image.llnl.gov
CC   Plate: LLCM2681  row: m  column: 04
CC   High quality sequence stop: 429.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..867
FT                   /db_xref="taxon:9606"
FT                   /note="Vector: pDNR-LIB; Site_1: SfiI (ggccattatggcc);
FT                   Site_2: SfiI (ggccgcctcggcc); Double-stranded cDNA was
FT                   prepared from a pool of 40 cell line polyA+ RNAs (bladder -
FT                   2%, blood - 33.4%, brain - 5.6%, breast - 12.5%, colon -
FT                   4%, connective tissue - 1.4%, eye - 1%, intestine - 2.6%,
FT                   kidney - 2.2%, liver - 5.7%, lung - 10.8%, NK-cell - 5.2%,
FT                   ovary - 4%, pharynx - 2.5%, prostate - 4.3%, salivary gland
FT                   - 1.3%, and skin - 2.3%). 5' and 3' adaptors were used in
FT                   cloning as follows:
FT                   5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' and
FT                   5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)NN-3'. Full-length
FT                   enriched library was constructed using the Clontech Creator
FT                   SMART kit and size-selected to contain the >0.5 kb size
FT                   fraction (other fractions present in NIH_MGC_141). Library
FT                   created in the laboratory of M. Brownstein (NIMH, NIH).
FT                   Note: this is a NIH_MGC Library."
FT                   /organism="Homo sapiens"
FT                   /clone="IMAGE:6498699"
FT                   /clone_lib="NIH_MGC_142"
FT                   /tissue_type="mixed (pool of 40 RNAs)"
FT                   /lab_host="DH10B (T1-phage-resistant)"
XX
SQ   Sequence 867 BP; 198 A; 221 C; 266 G; 182 T; 0 other;
     gggcctttca gcggagcgcg gcggcaacat ggcagtgcaa atatccaaga aggggaagtt        60
     tgtcgctgat ggcatcttca aagctgaact gaatgagttt cttactcgcg agctggctgc       120
     ggatggctac tctggagttg aggtgcgagt tacaccaacc aggacagaaa tcattatctt       180
     agccaccaga acacagaatg ttcttggtga gaagggccgg cggattcggg aactgactgc       240
     tgtagttcag aagaggtttg gctttccaga gggcagtgta gagctttatg ctgaaaaggt       300
     ggccactaca ggtctgtgtg ccattgccca ggcagagtct ctgcgttaca aactcctagg       360
     agggcttgct gtgcggaggg cctgctatgg tgtgctgcgg ttcatcatgg agagtggggc       420
     caaaggctgc gaggttgtgg tgtctgggaa actccgagga cagagggcta aatccatgaa       480
     gtttgtggat ggcctgatga tccacagcgg agaccctgtt aactactgac gttgacactg       540
     ctgtgcgcca cgtgttgctc agaccgggtg tgctggggca tcaaggttga agatcatgct       600
     gccctgggga cccaactggt aaagatgggc cctaaagaag ccccttgcct gaccaccgtg       660
     aggcattgtc ggaaccccca agatgaagat acttgcccac ccacccccca tttccagaac       720
     agaaaggggt gggaaaaccc aaagcccgcc ctgcgcaatg cccccaggcc aggtcccccc       780
     caggaataaa ccagggggtc tccccctttg ggcaacctgg taactcccgg ggagaatcct       840
     ggggactggg ttggccttcc tcctaac                                           867
//