LOCUS       BU546859                 563 bp    mRNA    linear   EST 16-SEP-2002
DEFINITION  GM880007A21C10 Gm-r1088 Glycine max cDNA clone Gm-r1088-2420 3',
            mRNA sequence.
ACCESSION   BU546859
VERSION     BU546859.1  GI:22929720
KEYWORDS    EST.
SOURCE      soybean.
  ORGANISM  Glycine max
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
            Spermatophyta; Magnoliophyta; eudicotyledons; core eudicots;
            Rosidae; eurosids I; Fabales; Fabaceae; Papilionoideae; Phaseoleae;
            Glycine.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Vodkin,L., Shoemaker,R., Keim,P., Retzel,E., Khanna,A., Shealy,R.,
            Clough,S., Thibaud-Nissen,F., Coryell,V., Erpelding,J., Raph,C.,
            Shoop,E., Stromvik,M., Schweitzer,P., Gong,G. and Liu,L.
  TITLE     A Functional Genomics Program for Soybean (NSF 9872565) (2002)
  JOURNAL   Unpublished (2002)
COMMENT     Other_ESTs: AW759433 corresponding to Gm-c1027-4157 (5')
            Contact: Vodkin, L.O., PI, A Functional Genomics Program for
            Soybean (NSF 9872565)
            Lewin, H. A., Director, Keck Center for Comparative and Functional
            Genomics
            University of Illinois
            Edwin R. Madigan Building, 1201 W. Gregory, Urbana, IL 61801, USA
            Tel: (217) 244-6147
            Fax: (217) 333-4582
            Email: l-vodkin@uiuc.edu
            Insert Length: 563   Std Error: 0.00
            Plate: GM880007A21  row: C  column: 10
            Seq primer: 5'-TTTTTTTTTTTTTTTTTTTT(A/C/G)-3'
            High quality sequence stop: 563.
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Glycine max"
                     /db_xref="taxon:3847"
                     /clone="Gm-r1088-2420"
                     /clone_lib="Gm-r1088"
                     /note="The library Gm-r1088 is a sequence-driven, reracked
                     set of 9,216 low redundancy clones selected from cDNA
                     libraries from various tissues and stages of development
                     of soybean that consists of 2,706 cDNAs from germinating
                     cotyledons (source library Gm-c1027); 1,355 cDNAS from
                     immature seed coats (libraries Gm-c1019 and Gm-c1023); 917
                     cDNAs from tissue culture derived somatic embryos (source
                     libraries Gm-c1036 and Gm-c1075); 3,275 cDNAs from
                     germinating seedlings, shoot tips, or leaves exposed to
                     various stresses (source libraries Gm-c1064, Gm-c1065,
                     Gm-c1066, and Gm-c1067; and Gm-c1068); and 963 cDNAs from
                     young leaves exposed to bacterial and fungal pathogens
                     (source libraries Gm-c1072, Gm-c1073, and Gm-c1074). The
                     5' ESTs of the source clones from the different libraries
                     was used to select singletons, or a representative of each
                     contig, which were reracked to form library Gm-r1088 and
                     the cDNA clones of the reracked Gm-r1088 library were then
                     sequenced at the 3' end. The unigene selection and 3'
                     sequencing was funded by NSF Plant Genome project #9872565
                     (http://soybeangenomics.cropsci.uiuc.edu/) as part of
                     creation of a low redundancy soybean cDNA set. The source
                     cDNA libraries were constructed by the laboratories of
                     Lila Vodkin, University of Illinois, Randy Shoemaker, Iowa
                     State University, and Paul Keim, Northern Arizona
                     University as part of the Public EST project,
                     http://129.186.26.94/soybeanest.html. The contig analysis
                     to select unique genes was performed by the laboratory of
                     Ernest Retzel, Center for Computational Genomics and
                     Bioinformatics, University of Minnesota,
                     http://web.ahc.umn.edu/biodata/nsfsoy/. Reracking and 3'
                     sequencing were conducted by services of the University of
                     Illinois Keck Center for Comparative and Functional
                     Genomics http://www.biotech.uiuc.edu/keck.htm. Note: The
                     corresponding 5' EST from each clone in the Gm-r1088
                     library is listed in the 'OTHER EST' field. The detailed
                     information on the source library for each clone can also
                     be obtained by referring to the Genome Systems clone ID of
                     the original cDNA library that is also listed under 'OTHER
                     EST'."
BASE COUNT      183 a    129 c    102 g    146 t      3 others
//