LOCUS       BU569087                 708 bp    mRNA    linear   EST 16-SEP-2002
DEFINITION  AGENCOURT_10360042 NIH_MGC_82 Homo sapiens cDNA clone IMAGE:6616416
            5', mRNA sequence.
ACCESSION   BU569087
VERSION     BU569087.1  GI:22919387
KEYWORDS    EST.
SOURCE      human.
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 708)
  AUTHORS   NIH-MGC http://mgc.nci.nih.gov/.
  TITLE     National Institutes of Health, Mammalian Gene Collection (MGC)
  JOURNAL   Unpublished (1999)
COMMENT     Contact: Robert Strausberg, Ph.D.
            Email: cgapbs-r@mail.nih.gov
            Tissue Procurement: CLONTECH
             cDNA Library Preparation: CLONTECH Laboratories, Inc.
             cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
             DNA Sequencing by: Agencourt Bioscience Corporation
             Clone distribution: MGC clone distribution information can be
            found through the I.M.A.G.E. Consortium/LLNL at:
            http://image.llnl.gov
            Plate: LLCM2860  row: a  column: 24
            High quality sequence stop: 192.
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:6616416"
                     /clone_lib="NIH_MGC_82"
                     /lab_host="DH10B (T1 phage-resistant)"
                     /note="Organ: testis; Vector: pDNR-LIB (Clontech); Site_1:
                     SfiI (ggccgcctcggcc); Site_2: SfiI (ggccattatggcc); 5' and
                     3' adaptors were used in cloning as follows: 5' adaptor
                     sequence:  5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence:
                     5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A,
                     C, or G and N = A, C,  G, or T).   Average insert size
                     1.35 kb (range 0.9-4.0 kb).  14/15 colonies contained
                     inserts by PCR. This library was enriched for full-length
                     clones and was constructed by Clontech  Laboratories (Palo
                     Alto, CA)."
BASE COUNT      295 a     87 c    159 g    163 t      4 others
//