LOCUS       BI882463                 175 bp    mRNA    linear   EST 16-SEP-2002
DEFINITION  fm93h04.x1 Zebrafish Research Genetics C32 fin Danio rerio cDNA
            clone 4468470 3' similar to SW:CAPB_HUMAN P47756 F-ACTIN CAPPING
            PROTEIN BETA SUBUNIT ;, mRNA sequence.
ACCESSION   BI882463
VERSION     BI882463.1  GI:16089734
KEYWORDS    EST.
SOURCE      zebrafish.
  ORGANISM  Danio rerio
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes
            ; Cyprinidae; Danio.
REFERENCE   1  (bases 1 to 175)
  AUTHORS   Clark,M., Johnson,S.L., Lehrach,H., Lee,R., Li,F., Marra,M., Eddy
            ,S., Hillier,L., Kucaba,T., Martin,J., Beck,C., Wylie,T., Underwood
            ,K., Steptoe,M., Theising,B., Allen,M., Bowers,Y., Person,B.,
            Swaller,T., Gibbons,M., Pape,D., Harvey,N., Schurk,R., Ritter,E.,
            Kohn,S., Shin,T., Jackson,Y., Cardenas,M., McCann,R., Waterston,R.
            and Wilson,R.
  TITLE     WashU Zebrafish EST Project 1998
  JOURNAL   Unpublished (1998)
COMMENT     Contact: Stephen L. Johnson
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: zbrafish@watson.wustl.edu
            cDNA Library Preparation: Ning Wu. cDNA Library Arrayed by: Steve
            Johnson. DNA Sequencing by: Washington University Genome Sequencing
            Center NOTE: This clone is available royalty-free through LLNL;
            contact the IMAGE Consortium (info.llnl.gov)for further
            information.
            Trace considered overall poor quality
            Seq primer: T7 from Gibco
            High quality sequence stop: 1.
FEATURES             Location/Qualifiers
     source          1..175
                     /organism="Danio rerio"
                     /db_xref="taxon:7955"
                     /clone="4468470"
                     /clone_lib="Zebrafish Research Genetics C32 fin"
                     /tissue_type="Fin"
                     /lab_host="GeneHogs (HS996, a phage-resistant isolate of
                     DH10B)"
                     /note="Vector: pT7T3D-Pac with a modified polylinker;
                     Site_1: EcoRI; Site_2: NotI; 1st strand cDNA was prepared
                     from zebrafish(C32) fin, and was then primed with a Not I
                     - oligo(dT) primer. Double-stranded cDNA was ligated to
                     Eco RI adaptors (Pharmacia), digested with Not I and
                     cloned into the Not I and Eco RI sites of the modified
                     pT7T3 vector.  Library is non-normalized. Library was
                     constructed by Ning Wu. NOTE: This clone is available
                     royalty-free through LLNL; contact the IMAGE Consortium
                     (info.llnl.gov)for further information"
BASE COUNT       31 a     51 c     47 g     46 t
ORIGIN      
        1 agccgtctcc agccttcttg atgaggatga cgccggcgaa ccagtggtcc agatcccaca
       61 ggtagacgga cgagaccccg tcctcgaagt acaggtctct gatctggtcg aaggcgttgt
      121 tggcctactc cttctccttt cttaggcgag ctgtcggcat tgttttctca tcgat
//