LOCUS       BI882696                 406 bp    mRNA    linear   EST 16-SEP-2002
DEFINITION  fm96e05.x1 Zebrafish Research Genetics C32 fin Danio rerio cDNA
            clone 4468689 3' similar to SW:RS8_HUMAN P09058 40S RIBOSOMAL
            PROTEIN S8. ;, mRNA sequence.
ACCESSION   BI882696
VERSION     BI882696.1  GI:16089967
KEYWORDS    EST.
SOURCE      zebrafish.
  ORGANISM  Danio rerio
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes
            ; Cyprinidae; Danio.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Clark,M., Johnson,S.L., Lehrach,H., Lee,R., Li,F., Marra,M., Eddy
            ,S., Hillier,L., Kucaba,T., Martin,J., Beck,C., Wylie,T., Underwood
            ,K., Steptoe,M., Theising,B., Allen,M., Bowers,Y., Person,B.,
            Swaller,T., Gibbons,M., Pape,D., Harvey,N., Schurk,R., Ritter,E.,
            Kohn,S., Shin,T., Jackson,Y., Cardenas,M., McCann,R., Waterston,R.
            and Wilson,R.
  TITLE     WashU Zebrafish EST Project 1998
  JOURNAL   Unpublished (1998)
COMMENT     Contact: Stephen L. Johnson
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: zbrafish@watson.wustl.edu
            cDNA Library Preparation: Ning Wu. cDNA Library Arrayed by: Steve
            Johnson. DNA Sequencing by: Washington University Genome Sequencing
            Center NOTE: This clone is available royalty-free through LLNL;
            contact the IMAGE Consortium (info.llnl.gov)for further
            information.
            Trace considered overall poor quality
            zebrafish identity (p-value greater than 1e-99) found to: TIGR:
            TC306 NULL
            Seq primer: T7 from Gibco
            High quality sequence stop: 1.
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Danio rerio"
                     /db_xref="taxon:7955"
                     /clone="4468689"
                     /clone_lib="Zebrafish Research Genetics C32 fin"
                     /tissue_type="Fin"
                     /lab_host="GeneHogs (HS996, a phage-resistant isolate of
                     DH10B)"
                     /note="Vector: pT7T3D-Pac with a modified polylinker;
                     Site_1: EcoRI; Site_2: NotI; 1st strand cDNA was prepared
                     from zebrafish(C32) fin, and was then primed with a Not I
                     - oligo(dT) primer. Double-stranded cDNA was ligated to
                     Eco RI adaptors (Pharmacia), digested with Not I and
                     cloned into the Not I and Eco RI sites of the modified
                     pT7T3 vector.  Library is non-normalized. Library was
                     constructed by Ning Wu. NOTE: This clone is available
                     royalty-free through LLNL; contact the IMAGE Consortium
                     (info.llnl.gov)for further information"
BASE COUNT       87 a    111 c     93 g    115 t
ORIGIN      
        1 tgcctttctt ggctttaatc ttcctcaggt agaactccag ttcttggccc tcaaggacgt
       61 atccatcgcc cctgccgaac tgccctggtc tggatgaaat gcaagccaaa agcttgccct
      121 gcatgaactg ctcctccatc ccaggactga tctaggctgt tatactgcgc agactaaact
      181 tgttttggac cttcttggac ctcttattga acaggatttc ctcttcctca gggggcagtt
      241 taacttcctt cttactgcca agtgggaggg catagtgaga ctcataccac tgactgtgag
      301 gagtgctgtc caccataaca acacagctgt tgaccagggt cttgggtcta accacctcgt
      361 ggtttgaagc attgtagacc acattaatga tcctggcctt gcgggt
//