LOCUS       BU552627                 888 bp    mRNA    linear   EST 16-SEP-2002
DEFINITION  AGENCOURT_10333005 NIH_MGC_40 Homo sapiens cDNA clone IMAGE:6576437
            5', mRNA sequence.
ACCESSION   BU552627
VERSION     BU552627.1  GI:22902899
KEYWORDS    EST.
SOURCE      human.
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 888)
  AUTHORS   NIH-MGC http://mgc.nci.nih.gov/.
  TITLE     National Institutes of Health, Mammalian Gene Collection (MGC)
  JOURNAL   Unpublished (1999)
COMMENT     Contact: Robert Strausberg, Ph.D.
            Email: cgapbs-r@mail.nih.gov
            Tissue Procurement: DCTD/DTP
             cDNA Library Preparation: Rubin Laboratory
             cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
             DNA Sequencing by: Agencourt Bioscience Corporation
             Clone distribution: MGC clone distribution information can be
            found through the I.M.A.G.E. Consortium/LLNL at:
            http://image.llnl.gov
            Plate: LLCM2774  row: p  column: 05
            High quality sequence stop: 710.
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:6576437"
                     /clone_lib="NIH_MGC_40"
                     /tissue_type="carcinoma, cell line"
                     /lab_host="DH10B (phage-resistant)"
                     /note="Organ: prostate; Vector: pOTB7; Site_1: XhoI;
                     Site_2: EcoRI; cDNA made by oligo-dT priming.
                     Directionally cloned into EcoRI/XhoI sites using the
                     following 5' adaptor: GGCACGAG(G). Library constructed  by
                     Ling Hong in the laboratory of Gerald M. Rubin (University
                     of California,  Berkeley) using ZAP-cDNA synthesis kit
                     (Stratagene) and Superscript II RT (Life  Technologies).
                     Note: this is a NIH_MGC Library."
BASE COUNT      201 a    244 c    296 g    147 t
ORIGIN      
        1 gtcttcccgc gtccgccgat tcctcctcct tggtcgccgc gtccttggct ggcgtcagaa
       61 aaatggctac aaacttccta gcacatgaga agatctggtt cgacaagttc aaatatgacg
      121 acgcagaaag gagattctac gagcagatga acgggcctgt tgcaggtgcc tcccgccagg
      181 agaacggcgc cagcgtgatc ctccgtgaca ttgcgagagc cagagagaac atccagaaat
      241 ccctggctgg aagctcaggc cccggggcct ccagcggcac cagcggagac cacggtgagc
      301 tcgtcgtccg gattgccagt ctggaagtgg agaaccagag tctgcgtggc gtggtacagg
      361 agctgcagca ggccatctcc aagctggagg cccggctgaa cgtgctggag aagagctcgc
      421 ctggccaccg ggccacggcc ccacagaccc agcacgtatc tcccatgcgc caagtggagc
      481 ccccagccaa gaagccagcc acaccagcag aggatgacga ggatgatgac attgacctgt
      541 ttggcagtga caatgaggag gaggacaagg aggcggcaca gctgcgggag gagcggctac
      601 ggcagtacgc ggagaagaag gccaagaagc ctgcactggt ggccaagtcc tccatcctgc
      661 tggatgtcaa gccttgggat gatgagacgg acatggctca gctggaggcc tgtgtgcgct
      721 ctatccagct ggacgggctg gtctgggggg cttccaagct ggtgcccgtg ggctacggta
      781 tccggaagct acagattcag tgtgtggtgg aggaacacaa ggtgggggac agaccttgct
      841 gggagggagg aaattcccca agttttgagg gagcaccgtg ccacgagt
//