LOCUS       BU553321                 985 bp    mRNA    linear   EST 16-SEP-2002
DEFINITION  AGENCOURT_10242487 NIH_MGC_109 Homo sapiens cDNA clone
            IMAGE:6577679 5', mRNA sequence.
ACCESSION   BU553321
VERSION     BU553321.1  GI:22903593
KEYWORDS    EST.
SOURCE      human.
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 985)
  AUTHORS   NIH-MGC http://mgc.nci.nih.gov/.
  TITLE     National Institutes of Health, Mammalian Gene Collection (MGC)
  JOURNAL   Unpublished (1999)
COMMENT     Contact: Robert Strausberg, Ph.D.
            Email: cgapbs-r@mail.nih.gov
            Tissue Procurement: ATCC
             cDNA Library Preparation: Rubin Laboratory
             cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
             DNA Sequencing by: Agencourt Bioscience Corporation
             Clone distribution: MGC clone distribution information can be
            found through the I.M.A.G.E. Consortium/LLNL at:
            http://image.llnl.gov
            Plate: LLCM2778  row: c  column: 23
            High quality sequence start: 13
            High quality sequence stop: 560.
FEATURES             Location/Qualifiers
     source          1..985
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:6577679"
                     /clone_lib="NIH_MGC_109"
                     /tissue_type="teratocarcinoma, cell line"
                     /lab_host="DH10B (phage-resistant)"
                     /note="Organ: ovary; Vector: pOTB7; Site_1: EcoRI; Site_2:
                     XhoI; cDNA made by oligo-dT priming.  Directionally cloned
                     into EcoRI/XhoI sites using the following 5' adaptor:
                     GGCACGAG(G).  Library constructed by Ling Hong in the
                     laboratory of Gerald M. Rubin (University of California,
                     Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and
                     Superscript II RT (Life Technologies). Note: this is a
                     NIH_MGC Library."
BASE COUNT      194 a    316 c    280 g    195 t
ORIGIN      
        1 ccaaatggcg gattacccca gagcagcggg ggggcccggg ggccctggtg gccctgggat
       61 ggggaactgc ggcggcttcc gcggaggttt cggcagtggc atccggggcc ggggtcgcgg
      121 ccgtggacgg ggccggggcc gaggccgcgg agctcgcgga ggcaaggccg aggataagga
      181 gtggatgccc gtcaccaagt tgggccgctt ggtcaaggac atgaagatca agtccctgga
      241 ggagatctat ctcttctccc tgcctattaa ggaatcagag atcattgatt tcttcctggg
      301 ggcctctctc aaggatgagg ttttgaagat tatgccagtg cagaagcaga cccgtgccgg
      361 ccagcgcacc aggttcaagg catttgttgc tatcggggac tacaatggcc acgtcggtct
      421 gggtgttaag tgctccaagg aggtggccac cgccatccgt ggggccatca tcctggccaa
      481 gctctccatc gtccccgtgc gcagaggcta ctgggggaac aagatcggca agccccacac
      541 tgtcccttgc aaggtgacag gccgctgcgg ctctgtgctg gtacgcctca tccctgcacc
      601 caggggcact ggcatcgtct ccgcacctgg gccctaaaaa gctgctcatg aatggcttgg
      661 caatccaatg aattgcctac cccttcaggc ccgggggcct tcacctgccc accccttggg
      721 ccaaatttcc cccccaaggg cccaccttct ggattgccca ttttctcaaa gaaccttaac
      781 cagcttaccc tggaaccccc cgcaagcttt ctgggtaaga ggaagacctg gtattctccc
      841 ccccaaacgc actccccctc aatctacgag gtttctccct agcacacccc ctctacatca
      901 caaggaacca ccacatctca acagagttct ttccttgggc cagtccggac acctccaacg
      961 cgttctcact gtttctggga ccaaa
//