LOCUS       BU554132                 887 bp    mRNA    linear   EST 16-SEP-2002
DEFINITION  AGENCOURT_10368692 NIH_MGC_109 Homo sapiens cDNA clone
            IMAGE:6581381 5', mRNA sequence.
ACCESSION   BU554132
VERSION     BU554132.1  GI:22904404
KEYWORDS    EST.
SOURCE      human.
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   NIH-MGC http://mgc.nci.nih.gov/.
  TITLE     National Institutes of Health, Mammalian Gene Collection (MGC)
  JOURNAL   Unpublished (1999)
COMMENT     Contact: Robert Strausberg, Ph.D.
            Email: cgapbs-r@mail.nih.gov
            Tissue Procurement: ATCC
             cDNA Library Preparation: Rubin Laboratory
             cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
             DNA Sequencing by: Agencourt Bioscience Corporation
             Clone distribution: MGC clone distribution information can be
            found through the I.M.A.G.E. Consortium/LLNL at:
            http://image.llnl.gov
            Plate: LLCM2787  row: n  column: 05
            High quality sequence stop: 721.
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /clone="IMAGE:6581381"
                     /clone_lib="NIH_MGC_109"
                     /tissue_type="teratocarcinoma, cell line"
                     /lab_host="DH10B (phage-resistant)"
                     /note="Organ: ovary; Vector: pOTB7; Site_1: EcoRI; Site_2:
                     XhoI; cDNA made by oligo-dT priming.  Directionally cloned
                     into EcoRI/XhoI sites using the following 5' adaptor:
                     GGCACGAG(G).  Library constructed by Ling Hong in the
                     laboratory of Gerald M. Rubin (University of California,
                     Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and
                     Superscript II RT (Life Technologies). Note: this is a
                     NIH_MGC Library."
BASE COUNT      212 a    234 c    277 g    164 t
ORIGIN      
        1 gaacggacgg cgatggcgac ggtcgcagca aatccagctg ctgctgcggc ggctgtggcg
       61 gcggcagcgg cggtgactga ggatagagag ccacagcacg aggagctgcc aggcctggac
      121 agccagtggc gccagataga aaacggcgag agtgggcgag aacgtccact gcgggccggc
      181 gaaagctggt tccttgtgga gaagcactgg tataagcagt gggaggcata cgtgcaggga
      241 ggggaccagg actccagcac cttccctggc tgcatcaaca atgccacact ctttcaagat
      301 gagataaact ggcgcctcaa ggagggactg gtggaaggcg aggattatgt gctgctccca
      361 gcagctgctt ggcattacct ggtcagctgg tatggtctag agcatggcca gccacccatt
      421 gaacgcaagg tcatagagct gcccaacatc cagaaggtcg aagtgtaccc agtagaactg
      481 ctgcttgtcc ggcacaatga tttgggcaaa tctcacactg ttcagttcag ccataccgat
      541 tctattggcc tagtattgcg cacagctcgg gagcggtttc tggtggagcc ccaggaagac
      601 actcggcttt gggccaagaa ctcagaaggc tctttggata ggttgtatga cacacacatc
      661 acggttctcg atgcggccct tgagactggg cagttgatca tcatggagac ccgcaagaaa
      721 gatggcactt ggcccagcgc acagctgcat gtcatgaaca acaacatgtc ggaagaggat
      781 gaggacttca agggtcagcc aggcatctgt gggcctcacc aatctgggca acacgtggct
      841 tcatgaactc cggccctgca gtgccctcag caatggtgcc acaggcc
//