LOCUS       AF069384                  60 bp    DNA     linear   SYN 02-OCT-2002
DEFINITION  Synthetic construct Mus musculus clone EW18.6 Ig switch region
            sequence, junction at positions 29-30.
ACCESSION   AF069384
VERSION     AF069384.1  GI:6048385
KEYWORDS    .
SOURCE      synthetic construct.
  ORGANISM  synthetic construct
            artificial sequences.
REFERENCE   1  (sites)
  AUTHORS   Marcu,K.B., Banerji,J., Penncavage,N.A., Lang,R. and Arnheim,N.
  TITLE     5' flanking region of immunoglobulin heavy chain constant region
            genes displays length heterogeneity in germlines of inbred mouse
            strains
  JOURNAL   Cell 22 (1 Pt 1), 187-196 (1980)
  MEDLINE   81042303
   PUBMED   6775817
REFERENCE   2  (sites)
  AUTHORS   Dunnick,W., Wilson,M. and Stavnezer,J.
  TITLE     Mutations, duplication, and deletion of recombined switch regions
            suggest a role for DNA replication in the immunoglobulin
            heavy-chain switch
  JOURNAL   Mol. Cell. Biol. 9 (5), 1850-1856 (1989)
  MEDLINE   89313728
   PUBMED   2747637
REFERENCE   3  (bases 1 to 60)
  AUTHORS   Stavnezer,J., Bradley,S.P., Rousseau,N., Pearson,T., Shanmugam,A.,
            Waite,D.J., Rogers,P.R. and Kenter,A.L.
  TITLE     Switch recombination in a transfected plasmid occurs preferentially
            in a B cell line that undergoes switch recombination of its
            chromosomal Ig heavy chain genes
  JOURNAL   J. Immunol. 163 (4), 2028-2040 (1999)
  MEDLINE   99370008
   PUBMED   10438941
REFERENCE   4  (bases 1 to 60)
  AUTHORS   Stavnezer,J., Rousseau,N., Shanmugam,A. and Kenter,A.L.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUN-1998) Department of Molecular Genetics and
            Microbiology, Univ. of Massachusetts Medical School, 55 Lake Ave.
            N., Worcester, MA 01655-0122, USA
FEATURES             Location/Qualifiers
     source          1..60
                     /organism="synthetic construct"
                     /db_xref="taxon:32630"
                     /clone="EW18.6"
                     /focus
                     /note="isolated from stably transfected I.29mu B cell
                     line"
     source          1..29
                     /organism="Mus musculus"
                     /strain="BALB/c"
                     /db_xref="taxon:10090"
                     /chromosome="12"
     source          30..60
                     /organism="Mus musculus"
                     /strain="I/St"
                     /db_xref="taxon:10090"
                     /chromosome="12"
     misc_feature    1..29
                     /note="Ig switch mu region"
     misc_feature    30..60
                     /note="Ig switch alpha region"
BASE COUNT       13 a     11 c     24 g     12 t
ORIGIN      
        1 ggtgaactga gctgggctga gctggactga atgacctggg atgggctgac ctagactgga
//